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Although its positive effects are well-documented, the mechanisms by which EA influences gene expression through epigenetic pathways, particularly via microRNAs (miRNAs) that suppress gene translation, are not fully understood [70]

In fact, it is quite well documented in many species that the expression of GSTF and GSTU genes is often induced in response to environmental constraints

In many studies the synthetic Bn peptide agonist [d-Phe 6 , -Ala 11 , Phe 13 , Nle 14 ]Bn(614) and its d-Tyr 6 analog were used due to their high affinity for the GRPR (Mantey et al., 1997

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Overall, co-expression pull-downs offer a faster, scalable, and more reliable strategy for studying difficult proteinprotein interactions

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