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One g of each RNA sample was used to synthesize complementary DNA (cDNA) using the PrimeScript RT Reagent Kit (Takara, RR037A, Dalian, China), following the instructions provided by the manufacturer

Limitations of the study The normal levels were established using Finish RedCross samples from assumingly healthy volunteers upon blood donation, and clinical pathology samples were collected from Auria Biobank material

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The erucic acid levels were not high enough for high value as an industrial oil

Research suggests people with low B12 levels are more likely to experience migraine compared to those with higher levels

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