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Supernatants were transferred to a new collection tube and dried down (for subsequent glutathione analysis) while the protein pellets were resuspended in 100 l 50% trifluoroethanol (TFE)/50 mM ammonium bicarbonate (ABC) and processed for redox and quantitative proteomics

For example, regional cerebral glucose metabolism was lower in both opioid withdrawal and methadone maintenance subjects than in control subjects (107, 108)

For glutamine treatments, serial two-fold dilution of glutamine (maximum concentration 5 mM) was performed in full media or low nutrient media

Pmc4 fused to the IAA17 degron was expressed from the endogenous pmc4 gene locus

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All patients provided written informed consent, and synovial samples were collected during knee replacement or open reduction internal fixation for tibial plateau fractures