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For example, when cultured in selenium-deficient conditions, Jurkat cells (a human T cell model) exhibit increased spontaneous cell death [18], due to decreased expression of selenium-dependent antioxidant enzymes (i.e., selenoproteins) such as glutathione peroxidases (GPx) and thioredoxin reductases leading to excessive oxidative stress and eventually cell death

Both experimental groups (saline- and Chitosan@DOTAGA-treated) underwent a slight weight loss in the first few days, which was then normalized over the 14-day period

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In summary, the support of the IVM medium with metabolic enhancers of the OCM was able to increase the DNA methylation of oocytes, although this difference did not play a role in their ability to reach the MII stage, which was similar between the groups

Fluorescence microscopy revealed that Mcherry-tagged AoEgt1 and AoEgt2 appeared as punctate structures of varying sizes (Fig