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doi: 10.1101/cshperspect.a030304 310 BoucheryTLe GrosGHarrisN

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Then excess derivatizing agent was evaporated and dry residue was converted to MOX-TMS (trimethylsilyl) derivative by reacting with bis(trimethylsilyl) trifluoroacetamide with 10% trimethylchlorosilane (Regisil) at 60C for 20 min