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Ultrathin sections were prepared using an ultramicrotome (Leica EM UC7), then mounted on copper grids, and stained with uranyl acetate and lead citrate

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The main targets of the predicted drugs and our list of predicted essential genes had a higher median dependency probability (likelihood that the knock-out of a gene reduces cell growth or induces cell death) than targets of known anti-melanoma drugs (Fig

Any underlying conditions, such as anemia, that may be causing the drop in blood pressure will first need treatment