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Additionally, the fact that accumulated amino acids can be applied in other metabolic procedures, such as certain biochemical reactions or the synthesis of new proteins, may be the reason for the decrease in the content of some amino acids (Ospankulova et al., 2025)

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The half-life of common local anaesthetics ranges from 1.6 - 3.5 hours, with bupivacaine having the longest half-life at 3.5 hours