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Circulating iron uptake in the form of two ferric iron (Fe 3+ ) bound to transferrin (TF) is achieved by binding to transferrin receptor protein 1 (TFR1) and subsequently triggering endocytosis [70]

Kit Components Additional Materials Required Microplate reader capable of measuring absorbance at 405 nm Precision pipettes to deliver 2 L to 1 mL volumes Multi-channel pipettes to deliver 20 L to 200 L volumes Tubes to prepare sample dilutions Ultrapure water 50 mL conical tubes Protocatechuic acid (HClO 4 ) (PCA) Potassium hydroxide (KOH) Assay Procedure Summary Add 50 L of the diluted GSSG Standards or unknown samples to the 96-well microtiter plate

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ns: not significant)

Total triglyceride concentrations decreased, and HDL concentrations increased in both groups throughout the study up to week 3, but to a greater extent in the KD group than in the standard diet group

Besides, the N-terminal metal ion binding site of albumin can chelate free metal ions, which plays an important role in the antioxidant activity of albumin to a certain extent (103)