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The coverslips containing the cells were then washed with ice-cold PBS three times, fixed with 4% paraformaldehyde at 4 C for 30 min, permeabilized with Triton X-100 for 10 min, and subjected to three 5-min washes

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The water-free formula prevents oxidation, keeping the vitamin C stable and effective from start to finish

The strain must be able to withstand as much as possible the successive steps of the overall process of removing water from cells (filtration, kneading, extrusion, drying, packaging) that lead to a dry, stable state without deterioration