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Protein samples aliquots were prepared as described in the 2D Clean-up Kit (GE Healthcare Life Science, USA), and the pellet of the final step was solubilized in sample buffer containing 7 M urea, 2 M thiourea, 40 mM Tris (0.5 M, pH 8.5), 4% CHAPS, 65 mM DTT, and 1% IPG buffer

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175,176 STATs are phosphorylated and translocated into the nuclear compartment to transcriptionally upregulate specific genes, 177 often leading to cell proliferation or differentiation

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Pinna A, Ragaisyte I, Morton W, Angioletti-Uberti S, Proust A, DAntuono R, Luk CH, Gutierrez MG, Cerrone M, Wilkinson KA, Mohammed AA, McGilvery CM, Surez-Bonnet A, Zimmerman M, Gengenbacher M, Wilkinson RJ, Porter AE