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We accomplished sensitivity analysis to find the effect of each particular study on the overall estimation (Reference Tobias45)

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In renal epithelial cells, TRPV4 channel activation results mainly from hypotonic cell swelling, which suggests that TRPV4 acts as an osmosensor and could play a critical role in renal ischemic/reperfusion injury 41,42 and in autosomal recessive polycystic kidney disease 43

The Folin Ciocalteu reagent [8] however cannot be carried out unless the concentration of Nycodenz or iodixanol is less than 5% (w/v): this situation however can often be attained if the final assay volume is 1-2 ml and the volume of gradient fraction used is 50 l