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2019;20:e47563 Lee J, You JH, Kim MS, Roh JL

Agitation causes foaming and can denature the peptide

This double assessment is necessary as the TUNEL fluorescent reaction may generate ambiguous results

Similarly, pyroptosis, a caspase-1-dependent form of cell death, induces gasdermin-D pore formation in the plasma membrane, culminating in cellular swelling and rupture [87, 88]