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Nuclei were stained with 5 g/mL DAPI

The beads were collected and washed, and the bound proteins were eluted by Laemmli buffer containing 500 L 6 M urea, 25 L 200 mM DTT, and 25 L 500 mM IAA in the dark at 25C for 30 minutes

doi:10.1016/j.socscimed.2017.12.005

If a pregnant patient has a documented deficiency of one or more B vitamins or a condition like hyperemesis that prevents oral intake, the benefits of correcting the deficiency (preventing serious maternal and fetal complications) outweigh any minimal risk from the injection

ADP-ribose-specific chromatin-affinity purification for investigating genome-wide or locus-specific chromatin ADP-ribosylation