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doi: 10.1210/me.2011-1379 54 AielloAPandiniGSarfsteinRWernerHManfiolettiGVigneriRet al

Subcutaneous tissue and muscles were separated by blunt dissection with forceps, the dura mater was blotted dry with a sterile cotton swab, and CSF was collected using a sterile pulled capillary to penetrate the cisterna magna (through the dura mater, lateral to the arteria dorsalis spinalis) and immediately snap-frozen in liquid nitrogen and stored at 80 C

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Research should focus on identifying these and developing value-added products, promoting a circular economy

That variation is chemically real and it is small: it moves the copper mass fraction by less than a tenth of a percentage point, which is far inside the uncertainty introduced by salt form and residual water in an actual vial