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Elevated NAD+ levels activate sirtuins, particularly SIRT1, which are NAD+-dependent deacetylases that regulate numerous metabolic processes including fat oxidation, glucose metabolism, mitochondrial biogenesis, and cellular stress responses

It exhibits an IC 50 of approximately 1 M for NNMT inhibition 4 , with favorable pharmacokinetic properties

5-Amino-1MQ is an oral NNMT (nicotinamide N-methyltransferase) inhibitor that works by blocking an enzyme that depletes cellular NAD+ and SAM

The compounds effects on NAD+ metabolism may have implications for muscle biology given the importance of NAD+-dependent enzymes in muscle function

Supplementary Table S5)