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The mice were housed in cages placed in a quiet and temperature-humidity controlled room (22C1-2C) with a 12 hr light-dark cycle

(9) Yiding Bian, Mingming Deng, Jia Liu, Jiaye Li, et al (2024) The glycyl-l-histidyl-l-lysine-Cu2+ tripeptide complex attenuates lung inflammation and fibrosis in silicosis by targeting peroxiredoxin 6 Redox Biology, Volume 75, September 2024, 103237

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Our protocol adjustments for Fitzpatrick IV-V patients using the GHK-Cu stack include more conservative SkinPen depth settings, lower needle density on the first session, an extended recovery window between sessions compared to lighter skin types, consistent mineral sunscreen guidance beginning two weeks before the first session, and careful timing to avoid treating recently tanned skin

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