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176 TanQ.ZhangL.GrantJ.CooperP.TegederM

In nonbiological samples, Craig and Bartlett previously observed biphasic decay of MeHg in the presence of H 2 S in an aqueous reaction mixture, with an initial rapid decay and then a slow decay that were probably due to the formation of (MeHg) 2 S, which is a somewhat water-insoluble material, and evolution of DMeHg from the reaction mixture, respectively 27 , thus supporting the results of our cell-free study

Effect of bridging the two essential thiols of myosin on its spectral and actin-binding properties

Add a non-ionic detergent (Tween-20 or Triton X-100) to the washing buffer, in concentrations ranging 0.01-0.1%

Achieve cryopreservation in an automated or manual controlled rate freezing apparatus following standard procedures (approximately 1 degree C decrease per minute)