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The protocol is reported according to SPIRIT guidelines, modified through inclusion of estimand framework (30)

Thus there is a need to better elucidate the ASD etiology and find biomarkers for early diagnosis and subsequently develop disease-modifying treatment for ASD

Description of the transcriptomes of immune response-activated hemocytes from the mosquito vectors Aedes aegypti and Armigeres subalbatus

The lysate was vortexed for 5 min followed by another 5 min of sonication and the aqueous phase was cleared by centrifugation, transferred to a new microcentrifuge tube, and placed in a vacuum concentrator for 3 h