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Gene expression analyses showed that eight genes involved in GSH metabolism were correlated to GSH concentrations, 2 of which (gamma-glutamyltransferase 1 [GGT1] and thioredoxin reductase 3 [TXNRD3]) were also correlated to MTX resistance

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The Black Box warnings, full side effect list, and drug interaction details remain in the Safety Details section below

Animal studies suggest that high concentrations of 1,25(OH)2D are necessary to generate Tregs when supplemented, but this can lead to adverse side effects such as hypercalcemia

Code availability R code used for data analysis is available via the Open Science Framework at References Jelen L

After 2 h treatment period, the mixtures were centrifuged at 10,000 g for 1 min to precipitate the erythrocytes