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Target proteins were further purified by size exclusion chromatography using a Superdex 75 16/600 column (GE Healthcare) in 20 mM Tris pH 7.5, 250 mM KCl, 1 mM DTT

Paeonol inhibits acsl4 to protect chondrocytes from ferroptosis and ameliorates osteoarthritis progression

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The acidic and reducing environment in lysosomes results in the formation of ferrous (Fe 2+ ) species, which in turn may catalyze the decomposition of H 2 O 2 in the Fenton reaction and produce OH radicals

doi: 10.1007/s00018-015-2044-6 119 BaptistaPAndradeJP