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However, our understanding of subcellular NAD(P) redox dynamics is still remarkably incomplete, mainly due to a lack of techniques allowing specific monitoring in defined subcellular compartments in vivo

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Anion exchange elutions were polished by gel filtration using a Superose 6 Hiload 16/600 column (Cytiva) equilibrated in storage buffer (25 mM HEPES, pH 7.5, 500 mM NaCl, 0.5 mM TCEP, 5% glycerol)

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