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Furthermore, the constant degranulation of mast cells depletes the body's supply of the diamine oxidase (DAO) enzyme, which is responsible for breaking down histamine in the digestive tract

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After incubation for 3 days at 37 C in a humidified atmosphere of 5% CO 2 , cells were fixed with 0.05% glutaraldehyde, followed by staining with 1% crystal violet in 20% ethanol for plaque counting