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Trypsin digestion was diluted with 500 L water, pepsin digestion with 200 L ABC (100 mM)

International Journal of Molecular Sciences (2018) 2

Next, biosensors were dipped into wells containing the second antibody, in the presence of the first competing antibody (all at 100 nM), and binding was measured after 900 s of association

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4 Nasal saline, antihistamines, and cromolyn are safe but may be less effective

Following two washes with Perm/Wash and PBS/BSA solution, cells were resuspended in 80 l PBS/BSA and analyzed on an LSR-II flow cytometer with FACSDiva software (BD Biosciences)