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It is a well-known fact that in clinical conditions associated with general immune suppression and anergy, such as malnutrition, immunosuppressive treatment, malignancies and severe physical trauma, Type IV reactivity to recall antigens as well as primary sensitization to contact allergens like dinitrochlorobenzene can be dramatically impaired

Qiu, L., Ding, X., Zhang, Z

After the kinase reaction, ADP-Glo Reagent at an equal volume to that of kinase reaction was added to terminate the kinase reaction and consumes the remaining ATP

Hewson CK, Capraro A, Wong SL, Pandzic E, Zhong L, Fernando BSM, et al

This type of discrepancy has been observed previously, but remains mechanistically unexplained to date 32

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