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the freeze-drying process comprises the following steps, preparing a liquid and filling at the temperature of 20-30 , putting a sample into a freeze dryer, quickly reducing the temperature of a plate layer to-40 , preserving heat for 1h, when the temperature of a clapboard is-10 +/-2 , preserving heat for 1h, quickly reducing the temperature of the plate layer to-40 within 1h, preserving heat for 2h, transferring to a cold trap at-50 , vacuumizing to below 10Pa, firstly preserving the temperature of the plate layer to-5 for 4h, then heating the plate layer to 10 within 3h, when a product is at 0 , starting to increase the temperature of the plate layer to 40 , and when the temperature of the product is increased to 30 , preserving heat for 4 h

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The collected villi were further washed using a 70 m cell strainer placed into one well of a 6-well plate (Falcon, #353046) filled with PBS and gently pipetted