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Total yeast and molds were enumerated following ISO 7954:1987, where samples were incubated for up to 5 days at 25C on Yeast Extract Glucose Chloramphenicol Agar (YGC-A)

Enhances collagen production and skin elasticity

No obvious threonine phosphorylated GSTP was detected (Figure S4A in Supplementary Material)

Finally, the extract was collected and kept in a volumetric flask at 20 C until it was needed for the experiment, and the yield % was recorded

HochbaumDRHulshofLUrkeAWangWDubinskyACFarnsworthHCet al

Figure 6C clearly shows that the experimentally observed biomass yields for both cases are consistent with the scenario involving glucose-PTS