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By leucocyte-depletion of RBC, by inhibiting proteases with phenylmethyl sulfonyl chloride (PMSF), by surface- 125 I -iodinating young and old RBC, by extraction with Triton and addition of a second antibody it was demonstrated that preexisting immune complexes contained band 3 monomers and oligomers in samples from old but not young RBC (Lutz and Stringaro-Wipf, 1983)

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In addition, the clinical dosage, individual variation, insufficient sample size, improper selection of endpoint indicators and short follow-up time may all be potential factors for the failure of antiaging clinical trials

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