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Materials and methods Post-mortem brain samples Frozen post-mortem tissues were obtained from the NICHD Brain and Tissue Bank for Developmental Disorders at the University of Maryland, Baltimore, MD, USA, and from the Autism Tissue Program at the Harvard Brain Tissue Resource Center, Belmont, MA, USA

The only challenge in application of melanin in oils stabilization may be their insolubility in hydrophobic environments

Metabolite extraction from serum and tissues For metabolomics analysis, 500 L of extraction solvent (ACN: MeOH: H 2 O = 2:2:1, v/v/v) was added to 100 L of serum or 25 mg of tissue sample

doi: 10.1016/j.ogc.2012.10.002 39

Why cycles matter for epitalon Consider how peptide cycling works at the cellular level

Durmaz, L., Karagecili, H., & Gulcin,