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When mitochondria fully utilize ATP, free radicals are produced as end products

The cells were maintained in DMEM (Gibco, Thermo Fisher Scientific) supplemented with 10% heat-inactivated FBS at 37 C and 5% CO 2

On day 2, colonies were re-struck on LB chloramphenicol IPTG 1mM LB plates overnight at 30C

The above led us to test a blend of taurine, vitamin B6, B9, and B12 in humans, in a randomized, double-blind, 2-arm, cross-over study with 44 participants aged 2540 years old