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Oxidation: Methionine and tryptophan are the most oxidation-prone amino acids in peptides

Shaking the vial, using the wrong diluent, or injecting the water too forcefully can shear the amino acid bonds

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Breakthrough technologies typically require years to decades of research work to optimize the technology and collect enough data to prove efficacy and superiority, which in some cases can optionally be submitted to the FDA if there is sufficient financial backing to market a specific product or drug