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The faster wound healing observed in the RGD-GHK group can also be attributed to faster angiogenesis

After incubation, the culture medium was collected, centrifuged at 2,000 g for 10 min to remove cellular debris, and the supernatant was carefully harvested for subsequent cytokine quantification using enzyme-linked immunosorbent assays (ELISA) kits: Mouse TNF alpha SimpleStep ELISA Kit (ab208348

It promotes angiogenesis, collagen organization, and anti-inflammatory cytokine balance while maintaining endothelial integrity

Scientists should log all compound use in their research documentation and ensure proper labeling requirements are maintained throughout the research process

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