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The recommended dose for pregnant women is 2.6mcg per day and 2.8mcg per day for breastfeeding mothers

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In this step Lys 185 acts as a general acid, donating a proton to the C-3 carbonyl oxygen, facilitating the decarboxylation

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Chem Biol 18(6):752765 Soane L, Li Dai W, Fiskum G, Bambrick LL (2010) Sulforaphane protects immature hippocampal neurons against death caused by exposure to hemin or to oxygen and glucose deprivation