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Glutamine dependency on oxygen consumption Briefly, 3.0 10 5 BT549 cells were seeded in a final volume of 100 L (complete RPMI media) in an XF24 plate (Agilent) and allowed to adhere for 1 h at 37 C in a 5% CO 2 humidified incubator, when more 150 L of same media was added and cells incubated in equal conditions for another 15 h

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