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Methods Plasmids, adenovirus, antibodies and regents cDNAs for full-length or 1-270aa (NT domain) WT, C45A, K179A, C180A, C371A, D270A, I217N GSDME were synthesized and cloned into pCDNA3.1 vector, and adenovirus-vector-based WT and Cys residues mutant plasmids were all from Sangon Biotech (Shanghai, China)

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