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Once reconstituted, store in the refrigerator and use within a short period to maintain integrity

10.1016/j.acra.2021.06.003 139

10.1186/s12944-020-01286-8 71 KomatsuM.KandaT.UraiH.KurokochiA.KitahamaR.ShigakiS.et al (2018)

But understanding the math yourself means you can verify your calculations and catch errors before they happen

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For barcode-specific reporter activation within a complex barcoded population, the reaction was scaled up to transform 250 l of competent cells with 200 ng of the enzyme plasmid and 200 ng of the targeting gRNA plasmid