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Importantly, the sgRNAs used did not generate off-target mutations across the genome, as confirmed by whole-genome sequencing (Supplementary Fig

Korean J Physiol Pharmacol (2015) 19:43

Substrate and coupling reagents were either prepared fresh or thawed from small aliquots stored at 80 C for a maximum of two freeze-thaw cycles

Ruminococcaceae are capable of generating short-chain fatty acids, which could inhibit the growth and reproduction of pathogenic intestinal bacteria by impacting the intestinal pH, which is a prerequisite and guarantee for achieving a good growth performance in broilers (69, 70)

These injections are administered intravenously, which ensures faster absorption and more effective results compared to oral supplements