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The cells were incubated with the cell lysis buffer [50 mM Hepes pH 7.4, 150 mM NaCl, 10% (v/v) Glycerol, 1% (v/v) Triton X-100, 1 mM EDTA, 100 mM sodium fluoride, 2 mM sodium orthovanadate, 20 mM sodium pyrophosphate, 0.5 mM dithiothreitol (DTT), 2 mM phenylmethylsulfonyl fluoride (PMSF)] supplemented with cOmplete Protease Inhibitor Cocktail (Roche) and PhosSTOP (Roche) on ice for 1 h

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Front Pharmacol 14:1101068

J Biol Chem 277(17):1480114811 Epub 2002/02/16 Cohen-Kashi-Malina K, Cooper I, Teichberg VI (2012) Mechanisms of glutamate efflux at the blood-brain barrier: involvement of glial cells

Reconstitution in 0.1% acetic acid (10 mM) is common practice, as the peptide tends to aggregate in neutral aqueous solutions without an acidifying agent