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To determine cell viability, 3,000 HT-1080 or MDA-MB-231 cells were seeded in a 96-well plate with standard medium (10% FBS and 1% penicillinstreptomycin) around 20 h before treatment

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Notably, addition of hemin or pyruvate, a source for NAD+ did not impact these growth phenotypes, suggesting that amelioration of heme defect or respiration inhibition is not sufficient to overcome anti-proliferative effects of mitochondrial GSH depletion (Extended Data Fig

Standardized evaluation frameworks for redox responsiveness, degradation kinetics, and toxicity remain underdeveloped

doi: 10.1080/07391102.2020.1835717 303 EberleRJOlivierDSPaccaCCAvillaCMSNogueiraMLAmaralMSet al

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