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3T3 fibroblasts were maintained in culture as described previously [24] and plated in 2 cm 2 LAB-TEK II chambered cover glass (Nunc, Thermo Fischer Scientific, Waltham, MA, USA) for 5 days, 72 h, 48 h, 24 h, and 6 h before the experiment and treated after attaching as explained before

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DON treatment activates AMPK in cancer cells by increasing the AMP/ATP ratio and decreasing the expression of c-Myc proteins, thereby inhibiting glycolytic metabolism and suppressing cancer cell growth in vitro 145

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U100 insulin: 100 units per mL U40 insulin: 40 units per mL That means if you used a U40 syringe with U100 insulin, your dose would be off by 2.5 times which could be dangerous