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Afterward, sections were washed three times with TBS and incubated with ImmPRESS-AP anti-rabbit IgG (alkaline phosphatase) polymer detection reagent (Vector Laboratories: MP-5402) for 30 min at room temperature

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Treatment of HepG2 cells with different concentrations of NFT or DEM for 24 h led to a concentration-dependent increase in Nrf2 and GCLC protein levels, whereas no significant change was observed in the levels of the modifier subunit, GCLM (Fig