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All animal procedures performed in this study were reviewed and approved by the Institutional Animal Care and Use Committee of the Zhejiang University School of Medicine and were conducted in accordance with the guidelines of the International Association for the Study of Pain

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Similarly, our model identifies distinctive language markers for BPD, such as discussions of abandonment and attachment issues, which are consistent with the clinical literature 2

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A rotatory microtome was used to cut 5 m slices of each sample, which were subsequently placed on coated glass slides for H&E staining 49 or positive glass slides for immunohistochemical staining of P53 and caspase-3.The immune histochemistry protocol was followed according to the protocol of Petrosyan, et al

Whole-blood measurements are dominated by erythrocyte glutathione and require appropriate volume- or erythrocyte-based normalization, whereas plasma and serum contain much lower concentrations and are particularly sensitive to hemolysis and processing delay