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doi: 10.37290/ijpp2641-7197.15:7-21

It is important to supply the body with sufficient collagen, as it is the main component of connective tissue

Cell 92 , 735745 (1998)

Plates were washed twice with ice-cold PBS, scraped, pelleted 250 g at 4 C for 3 min and treated on ice for 5 min with 4 ml of ice-cold HLB + N buffer (10 mM Tris-HCl (pH 7.5), 10 mM NaCl, 2.5 mM MgCl 2 , 0.5% NP-40, 1x phosphatase inhibitor cocktail (Sigma, P0044) and 1x protease inhibitor cocktail (Sigma, P8340))