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Minimize repeated freeze-thaw cycles

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Pull back the plunger just a little to make sure you have not hit a blood vessel

In case of poor results with methylfolate, either mega doses of methlfolate should be given (up to 15 mg were used in studies) to provide sufficient methyl groups or SAM can be given directly

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however, hCG was not detected owing to a poor ionization in the ESI source and high heterogeneity of hCG isoforms, consistent with previously published studies ( 2.5.2 XRPD All XRPD experiments were performed using a Bruker AXS D8 Advanced diffractometer equipped with a copper X-ray tube and a Vntec position-sensitive detector