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Prepare three density gradient solutions of 1.03, 1.04 and 1.06 g/ml (equivalent to 5.4%, 6.4% and 10.3%, w/v iodixanol) by diluting Optiprep with Solution B (see Note 2)

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Gene expression was normalized to the mRNA expression level of -actin as an endogenous control, and fold changes were calculated between treatment and negative-treatment control samples

23 Fumarylacetoacetate (FAA) is the natural substrate of FAH, but FAH also uses succinylacetoacetate (SAA) as a substrate (Figure 1)