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Sections were mounted in VECTASHIELD Antifade Mounting Medium and observed under a KEYENCE BZ-X700 fluorescence microscope equipped with a digital camera and a Tile-Scan program (Figure 2e)

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Importantly, supplementation can **increase** measurable NAD+ metabolism: in a 2018 randomized, placebo-controlled crossover trial published in Nature Communications by Martens and colleagues at the University of Colorado Boulder (24 healthy middle-aged and older adults), 1,000 mg/day of the NAD+ precursor nicotinamide riboside (500 mg, twice daily) elevated NAD+ in blood cells by roughly 60% versus placebo over 6 weeks, with no serious adverse events reported

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