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STA-384) from Cell BioLabs, Inc., according to the manufacturer's instructions

Collapsing of isoforms, transcript annotation, and transcriptome quality assessment (script: collapse_annotate_clean_transcriptomes.sh, busco.sh) Highly similar isoforms generated from the Trinity assembly were further collapsed to keep only the longest isoform as a representative using CD-HIT 48 (parameters: -c 0.99 -G 0 -aL 0.3 -aS 0.3) as differential abundance estimation has been shown to be more accurate at the gene versus isoform level 49

In addition, as it is often dismissed by both the public and healthcare workers, patients with this condition are frequently stigmatized and may avoid seeking help