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doi: 10.1038/s41420-021-00529-6 [DOI] [PMC free article] [PubMed] [Google Scholar] 68

F., Jr, Holbrook, M., Gokce, N., Swerdloff, P

Next, cells in both the experimental wells and the control wells were fixed with 4% formaldehyde in PBS for 30 min and incubated without shaking at room temperature on the benchtop

An elevated ESR indicates the presence of elevated fibrinogen (acute-phase reactant seen in inflammation ) or elevated immunoglobulins (seen in multiple myeloma), which promote red blood cell aggregation, causing them to sediment more rapidly