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Cells were incubated with 2,7-dichlorodihydrofluorescein diacetate (10 M) in serum-free medium for 30 min at 37 C with 5% CO 2

Clinically, two male siblings with progressive ataxia treated with 200 mg daily riboflavin experienced reductions in ICARS scores by approximately 44 and 20% at 6 months, suggesting a positive response despite overall phenotypic variability (54)

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This approach simplifies administration while capturing the synergistic benefits of all three compounds