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Following overnight incubation with the primary antibodies, membranes were washed five times for 15 min in TBST with mild shaking

For experiments with IMVs and for the purification of EtfD, the membrane fraction was resuspended in 15 mL (assays) or 40 mL (protein purification) S-buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 20% (v/v) glycerol, 5 mM MgSO 4 , 5 mM benzamidine hydrochloride, 5 mM aminocaproic acid, 1 mM PMSF)

TB-500 contributes superior biomechanical outcomes including increased ultimate tensile strength, improved elastic modulus, and enhanced failure load in healed tissues, with the peptide accelerating muscle regeneration following traumatic injury through enhanced satellite cell activation, improved myofiber formation, and reduced inflammatory infiltration

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Successful dual kidney transplantation after hypothermic oxygenated perfusion of discarded human kidneys

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