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In-depth investigation into the tissue-specific functions of these parallel pathways in chondrocytes and their crosstalk with the main GPX4 pathway holds significant translational medical importance for developing novel OA treatment strategies (Figure 1)

Rac1 is a member of the Rac family of guanosine triphosphate (GTP) phosphohydrolases (GTPases), which bind both guanosine diphosphate (GDP) and GTP, leading to its inactivation or activation, respectively

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To clarify the lysate, it is typically centrifuged at high speed to pellet the insoluble material

Zhang X, Jin X, Consoli U, Zhao S, Jiang S, Roth J, Andreeff M